Study of the Effects of Electrochemical Aqueous Sodium Chloride Solution (Anolyte) on the Virus of Classical Swine Fever Virus. Mathematical Models of Anolyte and Catolyte as Types of Water

Abstract

The results of antimicrobial action of an acid (anolyte) solution in electrochemical activation of sodium chloride are provided. Mathematical models are made of an acid (anolyte) and alkaline (catholyte) solution in electrochemical activation of sodium chloride. Anolyte water is produced by electrolyzing salt in the anode chamber of an electrolytic cell. Catholyte water is produced by electrolyzing water in the anode chamber of an electrolytic cell. Under laboratory conditions cell culture and organ suspensions of Classical swine fever virus were treated with anolyte. By inoculating them with cell cultures quality (displayed) viral presence (presence of viral antigen) was reported using the immunoperoxidase technique. It was found that: anolyte did not affect the growth of the cell culture PK-15; viral growth in the infection of a cell monolayer with a cell culture virus was affected in the greatest degree by anolyte in 1:1 dilution and less by the other dilutions; viral growth in the infection of a cell suspension with cell culture virus was affected by anolyte in dilution 1:1 in the greatest degree, and less by the other dilutions; viral growth in the infection with a virus in organ suspension of a cell monolayer was affected by the anolyte in all dilutions applied.

In order to provide additional information about the antiviral activity of anolyte, and about the distribution of water molecules according to the energies of the hydrogen bonds, mathematical models are made of anolyte and catholyte (Ignatov, Mosin, 2013).

This review reports about the research on the structure of intermolecular water cyclic associates (clusters) with general formula (?₂?)_n and their charged ionic clusters [H⁺(?₂?)_n]⁺ and [OH^-(?₂?)_n]^- by means of computer modeling and spectroscopy methods as ¹?-NMR, IR-spectroscopy, DNES, EXAFS-spectroscopy, X-Ray and neurons diffraction. Computer calculation of polyhedral nanoclusters (?₂?)_n, where n = 3–20 is carried out. Based on this data, the main structural mathematical models describing water structure (quasicrystalline, continious, fractal, fractal-clathrate) were examined and some important physical characteristics were obtained. The average energy of hydrogen bonding among ?₂? molecules in the process of cluster formation was measured by the DNES method compiles -0.1067 ± 0.0011 eV. It was also shown that water clusters formed from D₂? were more stable, than the ones from ?₂? due to isotopic effects of deuterium. The distribution according to energies of the hydrogen bonds in anolyte and catholyte as a percentage of the molecules with a certain value of hydrogen bonds to the total amount. The measurement of (-E) is in the range of 0.08 to 0.14 eV.

Keywords: anolyte, cell culture, CSF virus, disinfection, hydrogen bond, water, structure, clusters.