Studying the Virucidal and Biocidal Effects of Electrochemically Activited Anolyte and Catholyte Types of Water on Classical Swine Fever Virus (CSF) and Bactrerium E. coli DH5

Abstract

This article outlines the results on the antimicrobial action of electrochemically activated water solutions (anolyte/catholyte). The two types of water solutions are produced in the anode and cathode chamber of the electrolitic cell, respectively. Under laboratory conditions a strain of E. coli DH5, as well as the cell culture and organ suspensions of сlassical swine fever virus (CSF) were treated with the anolyte and the catholyte. By inoculating them with cell cultures the viral presence (the presence of viral antigen) was measured using the immunoperoxidase technique. It was found that anolyte did not affect the growth of the cell culture PK-15; the viral growth in the infection of a cell monolayer with a cell culture CSF virus was affected in the greatest degree by the anolyte in 1:1 dilution and less by other dilutions; whereas the viral growth at the infection of a cell suspension with the cell culture of the CSF virus was affected by the anolyte in dilution 1:1 in the greatest degree, and less by other dilutions; whereas the viral growth at the infection with the CSF in suspension of the cell monolayer was affected by the anolyte in all applied dilutions. Unexpectedly, the stronger biocidal effect of the catholyte was observed when a strain of E. coli DH5 was treated by anolyte and catholyte, respectively. In order to provide additional data about the antiviral activity of the catholyte and the anolyte, and the distribution of H₂O molecules according to the energies of hydrogen bonds, the non-equilibrium energy spectrum (NES) and diferential non-equilibrium energy spectrum (DNES) of anolyte and catholyte were measured.

Keywords: anolyte, catholyte, cell culture, CSF virus, disinfection, NES, DNES