Cytogenetic Estimation of Broccoli Extract on Dexamethasone Treated Mice

Abstract

The current study was intended to evaluate the active compounds found in hydro-alcoholic extract of Brassica oleracea var. italica(broccoli), also to estimates the percentage of chromosomal aberrations (CAs) ,mitotic index (MI) and micronuclei (MN) formation in mouse bone marrow cells .The mice were divided into, negative group received (0.2 ml of  Distilled Water)intraperitoneally ,positive group I was given single dose of dexamethasone (0.4 mg/kg for 24 h) and positive group II (acute toxicity assay) administrated with (0.2 ml) of  high level dose (800 mg/kg) of extract for 5 days. Interaction groups treated with  different doses of extract (50,100,200,400 and 600 mg/kg) for 5 days in addition to a single ( 0.4 mg/kg) of dexamethasone per each dose of extract. High level dose (800mg/kg) of broccoli extract had show null genotoxicity for bone marrow cells of mouse. Dexamethasone showed significant effects (P< 0.05)  in reducing mitotic index and increased chromosomal aberrations and micronuclei  in mouse bone marrow cells (in vivo). Broccoli extract significantly increased MI and reduced (CA ,MN) (P< 0.05) especially at does (400 mg/kg).

Keywords- mitotic index, Micronuclei formation ,Chromosomal aberrations , Dexamethasone, Brassica oleracea var. italica (broccoli), hydro-alcoholic extract.