Prevalence and Antimicrobial Susceptibility Pattern of Salmonella Species Isolated from Human Blood Samples in Robe Hospital, Bale Zone,South East Ethiopia

Abstract

The occurrence of Salmonella was a global challenge in the public health and food production sectors. Salmonella infections were one of the major global public health problems. The present study investigated the prevalence and antimicrobial sensitivity of strains of Salmonella species isolated from human blood samples of the patients in and around regions of Robe Hospital during the period of March to June 2016. This study revealed the fact that the prevalence of Salmonella was more common on males (14.68%) than the females (10.42%). The total prevalence of Salmonella species in Robe Hospital sample were (25.1%). The age group more commonly prone to this disease ranged from 1-30 followed by 50-60. The Salmonella Species were isolated by using different Selective media such as, Xylose Lysine Deoxycholate (XLD), Brilliant Green Agar (BGA), Macconkey agar and Salmonella Shigella agar (SS). The plates which showed black colonies were characterized using the biochemical analysis and identified as Salmonella species. Different Commercial antibiotics: Chloramphenicol (C), Rifampin(R), Tetracycline (T), Kanamycin (K), Ciprofloxacin (Cip), Ceftriaxone (CRO), Gentamycin (CN), Doxycline(D) and Streptomycin(S)were used to identified the sensitivity of pattern of Salmonella species. The isolated Salmonella species showed more sensitivity to Ciprofloxacin 100%, Ceftriaxone,91%, Gentamicin 58% antibiotics. Other antibiotics Doxycyline, Kanamycin, Chloramphenicol, Rifampin, Streptomycin and Tetracycline showed intermediate to complete resistance against tested Salmonella species. The study provides valuable information to agencies and legislators involved in making policy decisions about the use of antimicrobials.

Keywords: Antibiotics, Antibiotic sensitivity test, Biochemical test, Prevalence, Salmonella species.

DOI: 10.7176/JMPB/63-05

Publication date: January 31^st 2020