Expression of Alkaline Phosphatase by periodontial tissue treated with topical application of VEGF and TGF-beta1

Abstract

Growth factors are able to regulate significant cellular events in tissue regeneration and repair in periodontium injury.VEGF is a key regulator of physiological and pathological angiogenesis,because it induces endothelial cell proliferation, stimulates angiogenesis and TGF-beta1 effects on cell growth, biological interactions and receptor binding abilities .Both factors maintain  periodontal health and affected on the expression of ALP by periodontal tissue.

The aim of the present study To identify the biological events for expression of ALP by application  of VEG and TGF-beta1 , in periodontial healing.

Eighteen male Albino rats were subjected for non surgical operation of distal sides of  both lower anterior teeth, right side was considered as experimental site treated with 1µm of a combination of VEGF and TGF  ,while left be the control and treated with 1µm of normal saline.

All the samples were studied immunohistochemistry for identification alkaline phosphatase at three periods 3,7,14 day postoperatively.

The results demonstrated a higher records of positive  gingival epithelial cell,stromal cell ,PDL cell and bone cell that expressed strong immune-reaction of  ALP in combined (VEGF &TGF-?1)group in comparison to control.

Conclusion .The present study suggested that using of a combination of VEGF and TGF in injured periodontium  is a powerful tool in regenerative  of periodontium tissue ,by strong expression of ALK by epithelial and connective cells..

Keywords: vascular endothelial growth factor;periodontium healing,alkaline phosphatase,transforming growth factor.

Abbreviations : vascular endothelial growth factor(VEGF),periodontal ligament (PDL), alkaline phosphatase (ALP), transforming growth factor(TGF).