Novel Natural Anti Gout Medication Extract from Momdica charantia

Abstract

Medicinal plants were evaluated for their Xanthine Oxidase (XO) inhibitory potential. Their aqueous extracts, prepared from used parts, were tested in vitro, at 100 ?g/mL concentration, for their inhibition potencies expressed as % inhibition of XO activity. Two of the test plants were found inhibition% activity of xanthine oxidase. Namely Zingiber officinat (81.56±3.76) , Momdica charantia (96.5±2.17).Total Phenolic of Momdica charantia (80.83±0.30) was more than Zingiber officinat (62.18±0.65).Fraction of Momdica charantia (coumarin) was achieved the highest activity (inhibition activity of xanthine oxidase 97%) based on analysis HPLC. Screening of xanthine oxidase activity by coumarin in terms of kinetic parameter revealed uncompetitive mode of inhibition where K_m 25Mm^-1,V_max 0.38 Mm min^-1.Data showed that coumarin (0.5 mg/kg) treatment cause significant reduction in the serum uric acid level of hyperuricemia in normal mice. These finding suggest that Momdica charantia extract possess prominent medicinal properties and can be exploited as natural drug to treat diseases associated with free radical formation , oxidative stress and xanthine oxidase activity.

Keywords : Gout , Allpurinol , Xanthine Oxidase , Momdica charantia