The Chlorophyll Production and Hydrogen Produced Potency by Phytoplankton Chaetoceros Calcitrans, Chlorella Vulgaris, Dunaliella Salina, and Porphyridium Cruentum

Abstract

The research about Determination of chlorophyll productivity and potency of hydrogen produced by phytoplankton Chaetoceros calcitrans, Chlorella vulgaris, Dunaliella salina, and Porphyridium cruentum have been done.  It used sea water that was added by Conway medium and vitamin as culture media on the fourth species of phytoplankton test.  Dry biomass of phytoplankton was extracted using acetone p.a. to take the chlorophyll.  Analysis  method  was  carried  out  by  the  sonication  extraction for short  and cheap  in the lysis cell of phytoplankton and UV/VIS spectrophotometer to determine chlorophyll concentration through the Richards and Thompson equation.  The result indicated that phytoplankton C. vulgaris produced the most amount of the chlorophyll and the potency of hydrogen are 72.541 µg/mL and 636,4 L/kg DW successively from the dry biomass 1,4187 g and the higher cell density 3.060 x 10⁴ cell/mL.  The amount of hydrogen indicated that C. vulgaris has potential as the source of renewable energy.

Keywords: Chlorella vulgaris, chlorophyll, energy, hydrogen, photosynthesis.