Occurrence of Anti- Gonadotropins Hormones Antibodies in Rabbits Immunized with Pseudomonas Aeruginos

Abstract

The roles of gonadotropins hormones in fertility are well documented. This report deals with the effect of rabbit anti-Pseudomonas aeruginosa immune sera binding and depleting human Leutinizing hormone (LH) and follicle stimulating hormone (FSH). Capturing ELlSA (Pull-down assay) was used to enumerate the depletion potential of immune sera. The finding indicated the presence of anti- hormones antibodies in anti-bacteria immune sera. Percent depletion of males anti-bacteria antisera for (LH) was 35.1 ± 9.6 in test versus 3.3 ± 5.8 control (P=0.001) and for (FSH) was 38.95± 7.96 in test versus 2.1± 1.70 in control (P=0.0001).

On the Other hand, the percent depletion of female anti-bacteria antisera for LH was 10.9±16.9 in test versus 0.6± 1.07 in control, and for FSH was 14.04± 21.79 in test versus 1.075 ± 1.07 for control.

The presence of anti-gonadotropins hormones antibodies generated through immunization with Pseudomonas aeruginosa implicates a role of the immune response to this bacterium in immune-mediated effect in human fertility process.

Keywords: Anti - Gonadotropins, Anti-Hormone antibody, fertility