Expression of TGFβ1 by Pulp Tissue of Human Permanent and Primary Teeth Capped by BiodentineTM

Abstract

Biodentine^TM,represented a novel bioactive tricalcium silicate cement ,that is introduced into dentistry. It was suggested to be biocompatible, having optimal working and setting time, excellent workability with superior adhesion to tooth structure. Biodentine^TM was proved to maintain pulp as a vital tissue and enhance dentinal bridging, as alternative to Mineral trioxide aggregate that can be provided with a better handling characteristics and shorter working time. Moreover, dental pulp cells have the potential to differentiate into odontoblast-like cells and enhanced reparative dentine.In the present study "Biodentine^TM" was directly applied on the dental pulp of cultured tooth of (36) human maxillary first premolars and (36) human maxillary first primary molar tooth . After various culture periods (2,14 and 28) days, the interaction of the material with dental pulp tissue was analyzed on tissue cultures. The effect of this material on TGFB1 expression on pulp tissue was studied by immunohistochemical investigation. The results illustrate that Biodentine^TM" induced mineralized foci formation early after its application as direct pulp capping material in permanent and primary teeth. The mineralization appeared beneath the reparative dentine. Biodentine^TM shows a highl significant increment of TGF- β1 expression by pulp cells (P< 0.01) in both permanent and primary teeth.Conclusions: When "Biodentine^TM" was applied as direct pulp capping material for permanent and primary teeth, it induced an early form of reparative dentine synthesis, probably due to a modulation of pulp cell for expression TGF-β1.

Keywords: Transforming growth factor(TGF), Biodentine, pulp cells