Immunomodulatory of Cordia myxa (L.) Aqueous Extract Fruit in Immunized Mice with Hydatid Cyst Fluid

Abstract

Human cystic echinococcosis caused by Echinococcus granulosus is one of most important and widespread parasitic zoonoses in the world. The present study was aimed to identify the immunomodulatory activity of aqueous extract of Cordia  myxa fruit since this plant considers one of medically important plants, which is widely used for treatment of numerous diseases, that correlate with the effectiveness of  immunized by hydatid cyst fluid antigen HCFAg. Forty Balb/c mice were divided into equal groups, first group was immunized with HCFAg, the second group was treated with aqueous extract of C. muxa fruit, the third  group was immunized  and treated, the fourth  group was  as a control. Delayed type hypersensitivity (DTH), Mitotic index (MI) and histopathological change in spleen in all groups were studied. A higher increase of thickness was showed in immunized mice and treated after 10 days of treatment with aqueous extract of C.myxa fruit after 10 days(3^rd group), that reached 1.23± after 3 hrs.The MI of bone marrow and spleen cells was significantly increased as a post immunized and treatment mice (88.57±1.40), (94.7±0.76) respectively in comparison with the other groups. Histopathological examination of spleen showed marked hyperplasia of lymphoid corpuscles, confused some times to form large follicle. The successive aqueous extract was found to stimulate cell mediated and immune responses in mice/

Keywords: HCF, C. myxa, DTH, MI