Biochemical Potential of Vitamin C as the Free Radical Scavenger and Increase Reduced Glutathione (GSH) in Brain of Female Rat Administered Combined Oral Contraceptives.
Abstract
Background
Several millions of women in reproductive age all over the world make use of combined oral contraceptive to prevent unwanted pregnancy and treat menstrual abnormalities.Most combined oral contraceptives has been shown to induce oxidative stress in tissues such as brain. Since vitamin c has been shown with high antioxidant probabilities in many studies. The present study was undertaken to assess the potential of vitamin c as the free radical scavenger and increase reduced glutathione (GSH) in brain of female rat administered combined oral contraceptives.
Method
The experiment was performed in 24 female Wistar rats weighing 180g - 250 g. The animals were divided into 4 groups: Group 1(control), group 2(Vitamin C only ) (150mg/kg body weight). group 3(Combined Oral Contraceptive only) (0.667mg/kg body weight)and group 4(Combined Oral Contraceptive and vitamin C only) . After completing the experiments, the brains of all animals were collected and homogenized for further biochemical determination of antioxidant enzyme activities and vitamin C level.Data were analyzed using two-way ANOVA test followed by the Bonferroni’s multiple comparison test for analysis of biochemical data using SPSS version 10.0 .
Results
It was observed that group 3 treated with combined oral contraceptives only showed decreased GSH activity accompanying with oxidative stress, indicating by, the increased MDA activity and The beneficial effects of vitamin C treatment were shown in group 4 treated with combined oral contraceptives and vitamin C only accompanying with reduced oxidative stress. The effects are likely in dose-dependent manner.
Conclusion
This study indicates that vitamin c is effective in decreasing oxidative stress in the brain and help recovery of damages in cognitive function caused by combined oral contraceptives
Keywords: Combined Oral Contraceptive,Vitamin C,Brain and antioxidant enzymes
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ISSN (Paper)2224-3186 ISSN (Online)2225-0921
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