Assessment the Immunohistochemical Expression of Wild BRAF and Mutant BRAFV600E in Iraqi Patients with Colorectal Carcinoma

Abstract

In the current study hundred ten of Iraqi patients with colorectal tumors were studied to evaluate the expressionof wild BRAF and BRAFV600E using Tissue microarray-Immunohistochemisty (TMA-IHC) technique. Of them,Ninety cases had colorectal carcinoma, and twenty had benign tumors. A group of twenty cases of non-specificcolitis and other twenty colonic biopsies with no significant pathology were also studied. Results of the studydemonstrated that BRAF expression was positive in 86.7% of carcinoma cases, and there was significantdifferences (P=0.038) in the expression of BRAF within tumor stages. Whereas BRAFV600E expression wasnegative in groups of adenoma, colitis and cases with no significant pathology compared to carcinoma group inwhich 13.3% showed positive expression. We found significant correlation between the expression ofBRAFV600E and the age (P=0.047), right sided colon tumor (P=0.041), and mucinous type carcinoma (P=0.021).And there was significant differences between wild BRAF and mutant BRAFV600E in age, female gender, rightsidedcolon tumor, tumor grade, and type of tumor (p=0.0001).Keywords: BRAF, mutant BRAF, CRC, prognostic markers in CRC.