Studying of Some Genetics Variables in Patients with Diabetes Mellitus Type I and Type II

Abstract

Been highlighted through this study to determine the change in the levels of certain standards genetic in people with Diabetes Mellitus both types I and II, contained the current study, 60 people from diabetes patients and by (30) patients of the type I and (30) patients of the type II who underwent some tests, as well as (30) people (healthy apparently) as a group control of the study were collected blood samples from the Hussein Hospital education (blood test unit) and the Hospital of Al-Hindia General (Emergency unit), the Laboratory Razi analyzes of disease in the province of Karbala during the time period from 01.12.2011 until 01.04.2012. DNA was extracted for both types of diabetes patients and healthy people to detect the presence of the mutation in the gene for HLA-DR3 by the technique of the Poly merase chain reaction (PCR). the results of molecular genetic presence of mutations in the gene for HLA-DR3 in both types of diabetes and high incidence of mutations in individuals descended from parents who have a family history positive for the development of injury and also increased the proportion of males higher than in females and also recorded the incidence of high in the young ages of the type I and reconstruction late in the type II.

Keywords: diabetes, HLA-DR3, type 1 diabetes