Antioxidant and Anticancer Activity of the Marine Sponge Clathria Basilana

Abstract

This study aims to determine antioxidant and anticancer activity of  Clathria basilana extracts. The sponges were collected from Tablolong - Kupang , East Nusa Tenggara , Indonesia . The sponges were extracted with methanol. The crude extract was partitioned by n - hexane. The antioxidant activity of sponge extracts were tested by DPPH method . Anticancer activity of sponge extracts were tested by MTT method. Fraction of methanol - water at concentration of 500 ppm showed antioxidant activity of more than 50 % , while the fraction of n - hexane at concentration of 500 ppm showed antioxidant activity only 10 % . The test results showed that the fraction of methanol - water and n - hexane had anticancer activity of more than 50 % at concentration of 125 ppm.

Keywords: Clathria basilana, extraction, fractination, antioxidant, anticancer.