The Role of Lipopolysaccharide and O-Antigen of Proteus Mirabilis in Urinary Stones Formation, In-Vitro Study

Abstract

The ability to induce stone formation in-vitro of Proteus mirabilis isolates was investigated using whole bacterial cell, lipopolysaccharide, and O-antigen of these isolates. The results showed that all three parts have the ability to form crystallization in artificial urine solution, which based on the differences in urease activity and chemical structure of LPS and O-antigen. The whole bacterial cell of isolate No. 6 (P. mirabilis O18) revealed significant differences (P˂ 0.05) in the ability to bind with calcium (251.5 µg/ml) and magnesium (75.4 µg/ml) compared with the whole bacterial cell of isolate No. 14 (P. mirabilis O3) and isolate No. 3 (P. mirabilis O16), where the calcium concentrations were 238.77 µg/ml and 227.12 µg/ml, respectively; and magnesium concentrations were 53.34 µg/ml and 37.68 µg/ml for isolates No. 14 and 3, respectively. In contrast, LPS and O-antigen of isolates No. 14 and 3 showed significantly differences (P˂ 0.05) in the ability to metal binding with calcium (25.77 µg/ml and 25.06 µg/ml), respectively, and magnesium (6.6 µg/ml and 7.5 µg/ml), respectively, compared with LPS and O-antigen of isolate No. 6, where the calcium and magnesium concentration were 18.03 µg/ml and 3.16 µg/ml, respectively.