Assessment of the Genotoxicity, Antigenotoxicity on Alloxan-Induce Diabetic in Mice by Micronuclus(MN) Assay

Abstract

Fenugreek (Trigonellafoenum-graecum ) plant has become interest subject because of its beneficial effects on human health, good nutritive value in addition it has antigenotoxic  effects therefore, the purpose of the present study was to investigate antigenotoxic  effects of alcoholic extract of Fenugreek seed at 200 mg /kg b.w  on mice bone marrow which treatmed with alloxan, 6-mercaptopurine anticancer at 200 mg /kg b.w by using micronuclus test at 5 day for all treatments. Cytogenetic study showed significant increasing at (p< 0.05) in Micronuclus number  MN in diabetic mice, anticancergroup or both when compared to the negative control. After 5day of alloxan treated mice with marked hyperglycemia (blood glucose),results recorded significant increase at (p< 0.05) in diabetic group ( group treated with alloxan) than anticancer in same time compared with control. Finally, alcoholic extracts of  Fenugreek seeds found significant reduced MN, blood glucose in all treated groups. These results indicate that fenugreek plant were one of the primary supplements used to support type I diabetics or insulin-dependent diabetes mellitus (NIDDM), in addition  it has active compounds which have important role on antigenotoxic, hyperglycemia  activity.

Keywords: Extracts,Fenugreek,Alloxan,Micronuclus assay ,anticancer,mercaptopurine.