Genetics Diversity Analysis of Progeny F3 Soybean (Glycine max L.) Tolerant Salinity Using Microsatellite Markers

Abstract

Salt stress is one of the abiotic stresses that significantly reduce the yield of soybean. One of the strategies of plant breeding to produce soybean varieties that are resistant salinity is using artificial crosses that aimed to combine the properties of both parent genetics. SSR molecular markers can help to identify the genetic distribution that brings the character of salinity resistant high yielding of soybean. The purpose of this study were to determine the diversity of the progeny of soybean saline-tolerant and high yielding, information level of polymorphism using four SSR markers, determined the distance genotype progeny soybean with the elders and information on the available genetic diversity for selecting progeny candidates for possible use in a soybean breeding program of saline-tolerant soybean. Studies of genetic distance of 60 progeny soybean and 8 female individual numbers (61-64 ) and male (65-68) used four primary SSR markers was conducted in the Laboratory of Biomolecular Sucofindo Seed Production. This research used seeds were planted in crops with salinity levels of 5-6 dS / m-1 in a glass house at the Faculty of Agriculture, University of Sumatera Utara. Methods of SSR markers was applied with four SSR primers, 60 progeny soybean hybridization and eight numbers of female and male elders. The results showed the fourth primer is polymorphism with PIC average of 0:58. Cluster analysis divides the two groups. Only individuals who were outside 52 male group of male elders. There were 59 females and the progeny soybean traits such as female parent and male. F3 progeny soybean derivative Anjasmoro hybridization with saline resistant genotypes potential for genetic improvement to produce high saline resistant crops in order to support the expansion planting to support the improvement of soybean production in Indonesia.

Keywords: soybean, progeny soybean hybridization,saline-tolerant, microsatellite markers