Production of Exopolysaccharides from Submerged Culture of Antrodia Camphorata S-29

Abstract

Antrodia camphorata is a unique mushroom of Taiwan, which has been used as a natural therapeutic ingredient in Traditional Chinese Medicine (TCM) for protection of diverse health related conditions. Polysaccharides produced from A. camphorata have attracted much attention of research due to cytotoxic activity and miscellaneous activities. In this paper, we report on the fermentation conditions species-specific exopolysaccharides (EPS) from A. camphorata in submerged culture. A favorable medium for EPS production was obtained only by single-factor experiment, where Glucose and Yeast-Extracts were identified to be the most suitable carbon and nitrogen sources, with the concentration of 40 g/L and 5.0 g/L respectively. Zinc sulphate was identified to be the best salt source with the concentration of 0.4g/l. Initial pH and inoculum size for mycelial growth and EPS yield were 6.0 and 15% respectively. The maximum EPS production was 0.474 g/L in shake-flask culture, which is higher than the baseline media that was 0.351 g/L. This study provides the baseline information about production conditions for this specific specie which is crucial data to know before any further studies as it determines the properties and quantity of the desired produced specie.

Keywords: A. camphorata; Exopolysaccharide; Sub­merged culture.