Molecular Genotyping of Cystic Echinococcosis in Different Intermediate Hosts using 12 S Mitochondrial DNA Gene

Abstract

In any domestic area, it is important to establish control and prevention strategy for the characterisation of parasite genotype. The purpose of this research is the characterisation of various Echinococcus granulosus isolates found in Iraq, by means of the DNA sequence of the mitochondrial 12S rRNA. To conduct the study, naturally infected Echinococcus granulosus from thirty lungs and livers of goats, cattle, camel and sheep were gathered from the slaughter- house in Kirkuk city , during the period of February and December 2013. For the genetic work, almost eighteen fertile cysts were used. The technique that is premeditated and tested, required two novel primer sets that precisely amplified the portion of the mitochondrial 12S rRNA gene of 2 Echinococcus granulosus genotypes named G6 and G1. One of the two sets of primer augmented a portion of 674 base sets from the G6 genotype. Whereas the second pair amplified a portion of 253 base sets from the genotype G1.

Keywords: Echinococcosis, Molecular genotyping, DNA gene