Phenolic Glycoside and Steroid from the Roots of Comberetum Paniculatum

Abstract

Combretum paniculatum has been used widely in ethnomedicine where its root is traditionally used around Kaffa Zone and other central highlands of the country for the treatment of various illnesses, including conjunctivitis, eye ailments, leprosy, chronic diarrhea, dysentery, flatulence, vomiting and liver. Phytochemical screening tests of the methanol root extract revealed the presence of steroids, alkaloids, terpenoids, phenols, flavonoids, saponins, tannins, glycosides and the absence of anthraquinones. Silica gel chromatographic separation of the methanol extract gave cholest-5-en-3-ol (1) and mutiniside (2) for the first time from the roots. Complete characterizations of the isolated compounds were done with the help of spectroscopic techniques (UV-Vis, IR, 1D NMR).

Keywords: Combretum paniculatum, phytochemical screening,cholest-5-en-3-ol, mutiniside