In vitro Regeneration and Proliferation of Maize (Zea mays L.) Genotypes through Direct Organogenesis

Odunayo Joseph, Olawuyi, Olugbenga, Dalamu, Olumayowa Mary, Olowe


The variability of in vitro regeneration and proliferation of maize genotypes POOLISSRQPMX, DTSR-WCO, TZLCOMP4C3, TZE COMP 3C2, EV99QPM, POP66 SR/ACR94-YQPM, DTSR-WC, SAMMAZ 19S-14DT and TZEE-YPOPSTRC4 was investigated. Explants were regenerated through direct organogenesis using 0.1 mg/l NAA + 2.0 mg/l BAP, 0.1 mg/l NAA + 2.0 mg/l KIN, 0.3 mg/l NAA + 3.0 mg/l BAP, 0.3 mg/l NAA + 3.0 mg/l KIN, 0.5 mg/l NAA + 4.0 mg/l BAP, 0.5 mg/l NAA + 4.0 mg/l KIN and MS medium only. The regenerated maize yielded the highest number of multiple shoots (1.70 cm) and shoot length (3.35 cm) on MS medium supplemented with 0.3 mg/l NAA + 3.0 mg/l BAP within 31 days. Genotype EV99QPM and DTSR-WCO had the highest number of shoots, rooting traits respectively.

Keywords: Maize, regeneration, hormones, variability, survival, character

DOI: 10.7176/JNSR/9-6-09

Publication date:March 31st 2019

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ISSN (Paper)2224-3186 ISSN (Online)2225-0921

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