Regulation and Expression of ELOVL Fatty Acid Elongase-5 Genes with Overfeeding in Goose Fatty Liver

Abstract

Goose liver was an important economic trait which can be affected by high carbohydrate diet contents.  So, fatty acid elongase ELOVL plays an important role in the synthesis of long-chain polyunsaturated fatty acids (LCPUFA).  We hypothesized that ELOVL5 are involved in goose fatty liver development. To address this, we determined the response of goose ELOVL5 gene to overfeeding and their expression in goose liver and primary hepatocytes with related factors (glucose, fatty acid and insulin). Overfeeding expression data indicated that ELOVL5 was significantly reduction after two days of overfed.  In primary hepatocytes data expression by quantities PCR was not affected by glucose and palmitate treatment while reduction expression by high level of insulin. Bioinformatics analysis of the sequence gene was indicated considerably conserved among avian species.

Keyword: Fatty liver, Goose, ELOVL fatty acid elongase-5, Overfeeding

DOI: 10.7176/JNSR/9-11-02

Publication date:June 30^th 2019