Isolation and Characterization of Biosurfactants Producing Bacteria from Oil Polluted Soil

Okore Chioma, Mbanefo Ogechukwu, Onyekwere Bright, Onyewenjo Simon, Abba-Father Chinyere


Bio-surfactants are structurally diverse group of surface-active molecules, synthesized by micro-organisms. Oil polluted soils were collected from four oil spilled areas of different automobile workshops in Owerri Imo State. The samples were enriched in nutrient agar and serially diluted and pour plated in a nutrient broth plates. Pseudomonas aeruginosa and Bacillus subtilis were isolated and identified from these four samples.  A, B, C, and D, were screened for biosurfactant production using haemolysis test and oil spreading techniques which showed the oil displacement in the plates. The biosurfactant was characterized by running the surfactant in TCL plates.  Among nine isolates (X1 – X9), X 4 and X 8 were the best sources for the production of bio-surfactant for both Bacillus subtilis and Pseudomonas aeruginosa.

Key words: Bacteria, Pseudomonas aeruginosa, Bacillus subtilis, biosurfactant,  oil polluted soil

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ISSN (Paper)2224-3186 ISSN (Online)2225-0921

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