Microbial Contaminants and Their Control Methods: A Review with Reference to Potato Tissue Culture

Lemma Tessema


No cell or tissue culture problem is as universal as that of culture loss due to contamination, so that microbial hazards cause drastic economic losses in the plant tissue culture industries. A wide range of microorganisms (filamentous fungi, yeasts, bacteria, virus and viroid) and micro-arthropods (mites and thrips) have been identified as contaminants in plant tissue culture. With this paramount importance of in-vitro culture contaminants, various research work has been conducted from different corners of the world. This review paper compiles such important information to share experiences within a single amassed document. Different research and review articles, proceedings, protocol notes, scientific notes, conference papers, case reports as well as case studies, research communications and technical reports are included in this paper. Hence, this article aimed to review and provide insights about microbial contaminants and their management techniques and thereby to document the possible attempts made by different scholars to mitigate microbial contamination under plant tissue culture. Moreover, the paper skim through the current advancements made on culture loss due to microbes, their control methods and hostile effects on regeneration capability of culture plants.

Keywords: Microbial contamination, bacteria, fungi, plant tissue culture, in vitro culture

DOI: 10.7176/JNSR/12-10-01

Publication date:May 31st 2021

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ISSN (Paper)2224-3186 ISSN (Online)2225-0921

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