Histological and Biochemical Alterations in the Pancreas of Diabetic Wistar Rats Treated with Garcinia Kola and Tetracarpidium Conophorum Extracts

Abstract

To ascertain the stated effects, phytochemical and LD₅₀ studies of the extracts were determined. Thirty-six adult male Albino Wistar rats weighing 180 – 200 g were divided into 6 groups (n =6). Diabetes mellitus was induced by single intraperitoneal injection of streptozotocin (45 mg/kg bw) reconstituted in citrate buffer pH 4.5, and rats with fasting blood glucose (FBG) ≥ 11.3 mmol/L after three days of induction were used for the study. Glibenclamide significantly (p < 0.05) reduced FBG from 22.10 ± 1.65 mmol/L to 4.48 ± 0.29 mmol/L, G. kola from 22.04 ± 4.06 mmol/L to 7.40 ± 2.41 mmol/L, T. conophorum from 14.26 ± 2.38 mmol/L to 5.98 ± 0.57 mmol/L, and combined from 17.54 ± 1.72 mmol/L to 11.58 ± 2.11 mmol/L. Glibenclamide and the extracts significantly increase the insulin levels. G. kola significantly (p < 0.05) lowered the urea and raised the chloride and creatinine levels. T. conophorum significantly (p < 0.05) reduced the urea and increased the sodium and chloride levels, while the combined treatment significantly lowered the urea and raised the sodium levels. Glibenclamide and the extracts significantly ameliorated the histological alterations in the pancreas but only the plant extracts significantly attenuated the alterations in the kidneys. It may be concluded that the combined administration of G. kola and T. conophorum extracts and their single treatments showed hypoglycaemic and nephroprotective effects.

Keywords:Albino Wistar rats, Streptozotocin, Glibenclamide, Garcinia Kola, Tetracarpidium conophorum, Pancreas, Kidney

DOI: 10.7176/JNSR/13-14-04

Publication date:July 31^st 2022