Antioxidant and AntiproliferativeEffects on Human Liver HePG2Epithelial Cells from Artichoke (Cynara scolymus L.) By-Products

Abstract

Cynara scolymus L.is a medicinal plant frequently used in traditional medicine for liver diseases. This study aimed to evaluate the antioxidant and Anti-tumer effect in human Liver HePG2 epithelial cells of artichoke. The antioxidant activity of the free phenolic of bracts methanolic extract, as measured with the DPPH^.freeradical scavenging activity and iron chelating showed significant antioxidant activity > 0.05  as well as yielded the largest quantity of  phenolics and flavonoids  compounds. A significant increase was observed in hepatocellular carcinoma cell line (HePG2) 78.3 % treated with free phenolic extract of bracts higher than that found in by the heart of artichoke 36.7%. This work showed that extracts with high antioxidant activity can be obtained from artichoke and artichoke by-products. Therefore, this study indicate that the free phenolic extract from bracts of Cynara scolymusL. might be of interest within the developing market of nutritional ingredients and is capable of yielding nutritional supplements with antioxidant and anticancer activities.

Key words: Artichoke by-products, antioxidant, antitumor, HepG2