ANTIMALARIAL AND ANTIBACTERIAL BIOACTIVITY OF LANGSAT (Lansium minahasae L.) BARK EXTRACT

Abstract

This research is focused to obtain the data of the phytochemical compounds class that is contained in langsatbark, obtain the data of malaria antiparasite activity of langsat bark extract in vitro, and obtain the data of langsatbark extract antibacterial activity. The plant sample is obtained from Minahasa and Southeast Minahasa. Bothwet and dry langsat bark extraction is conducted using maseration method. Phytochemical analysis usesHarborne and UV-Vis spectrophotometer method. The antimalarial activity analysis is conduted in vitro on P.falciparum culture, whereas the antibacterial activity uses diffusion method. The research result shows that dryLangsat bark extracts (KBLK) that are from Minahasa (KBLKMI) and Southeast Minahasa (KBLKMT) have %highest extract yield, compared with the wet Langsat bark extract (KBLB). The highest extract yield KLBKMTpercentage is 5.78% and the lowest is n-butanol:water fraction, that is 0,663%. The thouroughly detectedcontents of phytochemical classes (alkaloids, flavonoids, saponins, tannins, steroids, triterpenoids, anthocyaninand quinone) is found on the KLBK extract. The highest intencity is found on KLBKMT. The strongestantimalarial activity in the 1000 erythrocyte that is infected by plasmodium, stated with LC50, is found onKLBKMT extract, i.e. 57056,1 ?g/ml and the weakest is KLBBMT extract, i.e. 80813,8 ?g/ml. The strongestantibacterial activity is stated with the diameter of formed inhibition zone is KLBKMT extract, that its average is12.5 mm in all experiment isolates.Keywords: bark, Lansium minahasae L., antimalarial, antibacterial