Third Generation DNA Sequencing Technologies

Abstract

The first and second generation DNA sequencing (SGS) technologies revolutionized the field of genomics has led to developments beyond surprisingly large number of scientific studies. It has been effective on the understanding of whole living genome sequence, characterization of genomic DNA and DNA sequence variation and live genom sequence encodes information of which part of genom sides, additionally detection of methylated regions of the genome, determining the level of mRNA transcription and translation, fully understanding of the interactions between protein and DNA characterized by different isoforms of determined existing genes. However a new generation of DNA sequencing technologies has provided new opportunities to the current sequencing technologies by creating innovative and fertile ground for the study of genome sequencing. In parallel with the current second generation sequencing (SGS) technologies, in addition to the development of technology, the new generation of single molecule sequencing which is also the third-generation DNA sequencing (TGS) technologies have been rapidly developed. Some approaches which are developed on third generation DNA sequencing technologies shows that an advanced level of using technology and products provide to a rich and original information for researchers that cannot be obtained by experimental method on DNA sequencing. Third generation DNA sequencing technologies are shown to be capable of working with a lot of studies on whole genome of any organism, in less than a day, much less cost, complete, accurate and creating longer sequencing reads chains.

Keywords: DNA sequencing, Second generation DNA sequencing, Third generation DNA sequencing