Molecular Characterization of Grapevine Fanleaf Virus (GFLV) and Grapevine Rupestris Stem Pitting-Associated Virus (GRSPaV) Vineyards in the Kırsehir

Abstract

This study, was conducted to investigate the presence of Grapevine fanleaf virus (GFLV) and Grapevine rupestris stem pitting-associated virus (GRSPaV) which causes significant loss of yield and quality in vineyard areas. 49 specimens of 16 vineyards were collected from Kirsehir provinces and districts. Viral RNAs isolated from the samples were tested with viruse-specific primer sequences in a two-step RT-PCR test. RT-PCR analysis revealed that 49 samples collected were not infected with GRSPaV. The collected 49 samples, 5 were only found to be contaminated with GFLV. GFLV, was quite high in samples from Kaman (20.0 %), followed by Akcakent (10.0 %), Akpinar (8.3 %) and center (0.0 %). As a result, it was determined that GFLV is more common than GRSPAV in cases collected from Kırşehir provinces.