Assessment of Anti-İnflammatory Activities and Antioxidant Properties of Methanolic Extract of ‘Sideritis montana’

Abstract

In this study, methanolic extract of Sideritis montana was investigated on total phenolic and flavonoid content, antioxidant capacities, scavenging activities of hydrogen peroxide and hydroxyl radicals and DPPH free radical. In addition, the anti-inflammatory effect of the plant extract was studied. The extract of S.montana showed that equivalents of phenolic content (157.29 ± 3.71 mg GAE/g DW), flavonoid content (403.99 ± 0.92 mg QUE/g DW) and total antioxidant capacities as (154.47 ± 4.12 mM α-tocopherol acetate / g dry weight).Also, the amount of extract supplying 50% inhibition of DPPH was assayed (58.48 ± 1.30 mg.L-1). S.montana was more effective than BHT, which is a synthetic antioxidant in hydrogen peroxide scavenging. But, when the hydroxyl radicals were scavenged the situation was exactly opposite. S.montana plant extract was able to inhibit heat-induced protein denaturation, but this activity was lower than that of diclofenac sodium used as a positive control. It has been determined that Sideritis montana methanol extract with high antioxidant activity also has anti-inflammatory activity.

Keywords: Sideritis montana; antioxidant; DPPH; anti-inflammatory