Cu/Zn SOD Enzyme Immunoreactivity in the Stomach Tissue of Rats Feedind with Great Scallop (Pecten maximus)

Abstract

Bivalve seafood grown in Dardanelles is exposed to environmental pollution for years. Especially heavy metal salts have accumulated in the flesh of marine species. Great scallop is consumed as food and it is a valuable product. There are no studies on the effects toxic of other living organisms consuming bivalve marine life in the Dardanelles. In this study, it was aimed to investigate the effects of great scallop on Cu/Zn SOD enzyme production. The great scallop given as food to the rats were removed from the Dardanelles Çardak region. Four groups of rats are included in the study, group 1 (n=6), control group fed with standard rat food, group 2 (n=6), 75% great scallop and 25% standard rat food daily, group 3 (n=6), 75% great scallop and 25% standard rat food every two days, group 4 (n=6), 75% great scallop and 25% standard rat food every three days. To detect Cu/Zn SOD localization in the tissues, the LAB-SA Detection System was used. Cu/Zn SOD immunoreactivity was detected of epithelial cells in the gastric mucosa of rats fed with great scallop. Cu/Zn SOD enzyme immunoreactivity was detected in gastric mucosal epithelial cells of great scallop-fed rats. Cu/Zn SOD enzyme immunoreactivity was observed in 91% in the second group, 88.67% in the third group and 68.50% in the fourth group. We think that the reason for the detection of Cu/Zn SOD enzyme immunoreactivity in the gastric mucosa of rats fed with sea tark collected from Dardanelles is due to the accumulation of heavy metal salts in great scallos.

Key words: Immunohistochemistry, great scallop, Dardanelles, Cu/Zn superoxide dismutase, stomach