The Cytotoxic Effects of Titanium Oxide Nanoparticle on MDA-MB–231 AND MCF–7 Cells

Abstract

Breast cancer is one of the leading causes of mortality among women around the world due to various factors such as aggressive invasion, early metastasis, resistance to existing chemotherapeutic drugs, and high mortality. Because traditional chemotherapeutic agents affect the whole body system through the blood, there are too many systematic side effects such as tissue damage, gastrointestinal stress. In recent years, scientists have focused on research on nanotechnology molecules that exhibit anti-cancer activity, and progress has been made to the relevant pharmacotherapeutic field. Nanotechnology is one of the fastest developing research fields in the world and in our country. In this study, Titanium oxide (TiO2) nanoparticle was synthesized. The formation of TiO2 nanoparticles was characterized by UV–vis spectrophotometry and zeta potential measurements. X-ray diffractometer (XRD) spectrum of the nanoparticles confirmed the formation of TiO2 nanoparticles. The synthesized TiO2 were applied to the MDA-MB-231 and MCF-7 cancer cell line (1x105 cells/well, 96 well plates in DMEM supplemented with 10% FBS and 1% penicillin) and cytotoxic effect was determined. Cytotoxicity was carried out using (3- (4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT). MDA-MB-231 and MCF-7 cells were treated with different concentrations of TiO2 for 24h, 48h and 72 h. The spectrophotometric readings at 570 nm were analyzed by the Graphpad Prism7 program. MTT test results showed that TiO2 significantly reduced cell viability by compared to control.

Key words: TiO2, MDA-MB-231, MCF-7, Breast cancer, Nanoparticle