Determination of Antimicrobial Activity of Tyhme and Black Cumin Seed Essential Oils Against Some Clinical Isolates

Abstract

In this study, antimicrobial activities of commercial thyme-1, thyme-2 (Origanum onites and Thymus spp.) and black cumin seed (Nigella sativa ) essential oils, with ethanol, methanol, chloroform, acetone, aqueous extracts of thyme (Origanum vulgare ) and black cumin seed (Nigella sativa ) were investigated against different eleven clinical isolates. For this purpose, disc diffusion and microdilution methods were used in antimicrobial activity experiments. Minimum inhibitory concentration values of essential oils with significant antimicrobial activity were determined. According to the disc diffusion findings of the study; the highest inhibition zone values were obtained from commercial thyme-2 and thyme-1 (Thymus spp. and Origanum onites) essential oils, respectively. In addition, the minimum inhibitor concentration values of essential oils were obtained from thyme-2 ( Thymus spp.) and thyme-1 (Origanum onites) essential oils, respectively. It was determined that most of the extracts obtained from black cumin seed (Nigella sativa) and (Origanum vulgare) thyme and commercial black cumin seed (Nigella sativa) essential oil against clinical isolates were either not effective at all, or that their activities were lower than commercial thyme-2 and thyme-1 essential oils.

Keywords: Antibacterial Activity, Essential Oils, Pathogen Bacteria, Plant Extract.