Evaluation of Synergistic Effect of TiO2 and Al2O3 Nanoparticles on Hela Cell Line

Abstract

Objective: The aim of this study was to analyze and compare the cytotoxic effects aluminium oxide (Al2O3) and titanium dioxide (TiO2) nanoparticles (NPs) and binary mix on them on Human cervical cancer (CC) cell line (HeLa) and healthy mouse fibroblast cell line (L-929) in vitro. Materials&Methods: In the study, TiO2 nanoparticle was synthesized by sol-gel method. Al2O3 nanoparticle is commercially purchased. TiO2+Al2O3, Al2O3 and TiO2 nanoparticles were dissolved in water under certain conditions by probe and characterization analyzes were performed. Cytotoxicity of the TiO2+Al2O3, Al2O3 and TiO2 was evaluated by MTT assay. HeLa and L-929 cell line were treated with different concentrations of this nanoparticles (0,5-100 μg/ml) for 24, 48 and 72 hours. Results: In our study, TiO2+Al2O3, Al2O3 and TiO2 nanoparticles were prepared in water. UV-vis spectrofometric analysis and zeta potential measurements of this nanomaterials were performed. For this nanoparticles comparable cytotoxic action on HeLa and L-929 cell line were detected. The effects of TiO2+ Al2O3, Al2O3 and TiO2 nanoparticles on the HeLa and L-929 cell line were compared to the control group and IC50 values were found for 24, 48 and 72 hours. We also compared the effect of these nanoparticles on the HeLa cell line with the healthy L-929 cell line. The spectrophotometric readings at 570 nm were recorded and analysed with Graphpad Prism7. Conclusion: There are many drug methods for CC treatment. However, new treatment methods are needed to minimize unwanted side effects and to overcome drug resistance. In this study, we found that the HeLa cell line of TiO2+Al2O3, Al2O3 and TiO2 nanoparticles had anticancer effects and inhibited cell growth. We found that these drugs were more active in the HeLa cell line compared to the L-929 cytotoxic effects on the HeLa cell line.

Key Words: Cervical cancer, HeLa, L-929, TiO2, Al2O3