Investigation of the Cellular Behavior of Polycaprolactone-Hydroxyapatite Tissue Materials Produced with Bioprinter

Abstract

One of the most remarkable technologies for developing tissue engineering and artificial organ systems is 3D printers. 3D printers are a very fast developing technology that enables you to produce 3D solid objects from virtually virtualized data. Almost all 3D science printers used in the field of bioprinter, medical applications in the production of tissue and organ production has come forward. The first biological material printed with bioprinter is a supporting structure for bone tissue. In recent years, with the spread of this technology, live tissues such as cartilage, skin, heart valve and aortic vein have been produced. Bioprinter technology, organ transplantation related to the negativity of the tissue and organ regeneration are thought to be very important functions. In this study, tissue material production was produced by synthesized hydroxyapatite (MSSHA) bioceramics from seashells supplemented with polycaprolactone (PCL) matrix. MSSHA bioceramics were synthesized from sand shells (Donax trunculus) seashells by chemical agitation. Characterization studies of bioceramic and tissue scaffolds, structural (X-ray Crystallography (XRD), Fourier Transform Infrared Spectroscopy (FTIR), thermal (Thermal Gravimetric Analysis (TGA), Differential Scanning Calorimeter (DSC)), morphological (Field Emission Gun Scanning Electron Microscope) (FEGSEM), mechanical (Tensile) and biological (Cell Culture) analysis, based on the analysis values can be used in the major tissue engineering applications that can adapt to the body and biodegradable as alternative tissue material has been determined.

Keywords: Tissue Engineering, Seashells, Hydroxyapatite, Bioceramic, Polycaprolactone, Bioprinter, Tissue Material.