Determination of anti-cancer and apoptotic effects of Tricholoma terreum, Tricholoma arvernense and Tricholoma imbricatum species

Abstract

Macrofungi has been used for food and therapeutic purposes for centuries. In terms of their medicinal properties, the fungi contain a variety of polysaccharides, polysaccharide-protein complexes and secondary metabolites. The antimicrobial, antioxidant and anticancer effects are associated with these ingredients. As a cancer-associated fungus, Ganoderma lucidum is the best known in the world. On the other hand, anticancer activity and contents of many mushrooms were investigated. In the genus Tricholoma, T. matsutake is well known in Japan. The medicinal properties of this mushroom have been demonstrated. In this study Tricholoma terreum, Tricholoma arvernense and Tricholoma imbricatum from Turkey have been investigated for anticancer activity and apoptotic effects. Derived extracts from collected fungi were tested against HL 60 and MCF-7 human cancer cell lines. Antiproliferative effect were determined by using MTT method and determination of apoptotic cells was performed using Hoechst/Propidium iodide (HO/PI) double staining method. Different extracts from T.terreum, T. arvernense and T.imbricatum species inhibited 50% of the cells in the concentration range of 15-20 mg / ml, especially in the HL-60 leukemia cell line and in apoptosis research with these extracts T. imbricatum methanol extract caused about %60 apoptotic cell death at the 40 mg/ml concentration.

Keywords: Apoptozis, Tricholoma, Cell culture

DOI: 10.7176/JSTR/5-2-03