Usability of Some Reactive Dyes in Human Blood Cells (HBCs) Staining

Abstract

The application of Remazol dyes as a synthetic dye for textiles has been known for a long time; in addition, some synthetic dyes are also used for staining histological sections. These dyes have different chemical structures but they have the same chemical bonding principle with materials like cells or textiles. The aim of this study is to determine for Human Blood Cells (HBCs) through dyeing with some reactive dyes. For this purpose, we used four Remazol dyes of different colors like Rot R, Dark Blue, Schwarz RL, and Blue BB. Application of these dyes to HBCs on slides carried out at different times like 15, 30, 45, and 60 minutes and altering temperatures like 25°C, 60°C, and 100°C, respectively. The slides were then washed with distilled water, air dried and viewed at 1000 magnification by light microscopy (Leica microsystems D30). As a result, we found that, HBCs were dyed in pale and light color by all Remazol dyes at 100°C for 60 minutes with soda-sulfate mordant. HBCs partially denaturated at all temperatures and times. However, HBCs were dyed a darker red color at 100°C for 60 minutes by Remazol Rot R and alum. We conclude that Remazol Rot R, Dark Blue, Schwarz RL, and Blue BB dyes have potential for dyeing HBCs with alum at 100°C for 60 minutes.

Keywords: Human Blood Cells, Histological stains, Reactive dyes, Remazol, Textiles, Synthetic dyes

DOI: 10.7176/JSTR/5-2-07