Effect of Cerium on Erythrocyte Mechanical Properties

Abstract

Lanthanide’s are known as rare earth elements and are used in many areas. Although the place of Cerium in the Lanthanide family in its biological importance in mammalian physiology is not fully known, a human weighing 70 kg contains about 40 mg Cerium. Soluble Ce3+ salts (nitrate, acetate, chloride, etc.) are used for many biomedical purposes and in particular act as calcium analogues in biological systems. In non-physiological flow conditions occurring in extracorporeal circulation areas, increased calcium ions within the cell play a role in reducing erythrocyte deformability. In this study, it is aimed to show the possible effects of Cerium on changes in erythrocyte deformability in shear stress at sub-hemolytic level. For this purpose, blood samples were taken from adult male volunteers. Normal erythrocyte deformability was measured at nine shear rates between 0.5 and 50 Pa, followed by stress application at a slip rate of 290 Pa for 300 seconds without removing the sample from the system, followed by erythrocyte deformability at shear rates of 0.5-50 Pa. There was no significant difference between the erythrocyte deformability values of the control and Cerium incubated blood samples before stress application. However, significant differences were found after stress application (p <0.01). Incubation of erythrocytes with Cerium did not cause any change in erythrocyte deformability, but caused further deterioration of erythrocytes after mechanical stress. Cerium chloride, which we use in our study, should be kept in mind that under normal conditions, even if there is no negative effect on erythrocyte mechanics, conditions change, erythrocytes are exposed to short-term sub-hemolytic mechanical effects and their mechanical properties are deteriorated.

Keywords: Lanthanide, Cerium, Erythrocyte, Deformability, Mechanical Stress

DOI: 10.7176/JSTR/5-8-11