Effect of Seaweed Extract Applied to Growth Media on Mycelium Growth of Oyster Mushroom

Abstract

This study was carried out to determine the effect of Ecklonia maxima type seaweed extract applied to growth media on mycelium growth of oyster mushroom (Pleurotus ostreatus). In the experiment, most common 2 growth media [Malt Extract Agar (MEA) and Potato Dextrose Agar (PDA)] were used. After the prepared MEA (48 g L 1) and PDA (39 g L 1) medium was autoclaved at 121 ° C for 20 minutes, half was separated and 2% E. maxima seaweed extract (DY) was added. The experiment consisted of 4 growth media: MEA, PDA, MEA + DY and PDA + DY. While the prepared environments were warm and fluidal, they were filled in petri dishes under UV light in a sterile cabinet and left to cool. After surface disinfection of oyster mushrooms, clones were taken and placed on the medium in petri dishes and petri dishes were closed with parafilm. The petri dishes (n=15) were placed in the incubator at 24 °C. Daily mycelia lengths and weight of petri dishes were measured and mycelia growrth pwriod (day) and rate (cm day 1) were calculated. Among the used growth media, PDA was given more and heavier mycelia growth in a shorter time than MEA. DY application was increased mycelia growth period and reduced growth rate. DY was showed better performance in PDA medium. As a result of the research, it has been determined that the addition of liquid seaweed extract preparation to PDA at a rate of 2% (PDA + DY) increased micelle growth and shortened micelle growth period.

Keywords: PDA, MEA, Ecklonia maxima, Oyster mushroom

DOI: 10.7176/JSTR/7-03-02