Effects of Plant Growth Regulators on the In Vitro Cultures of Vitis vinifera L.

Abstract

Vitis vinifera L. (grape) is a plant that is cultivated extensively in the world and in our country because of it’s economic importance. Grape fruit can be use as juice, molasses, wine and medicinal industry. Although there are many types of genetically potent grape varieties, today there is a growing demand for varieties with high yield and quality, especially resistant to stress factors. The aim of our research were comparison of MS and Gamborg nutrient mediums on in vitro grape callogenesis levels. In this research, petiole and shoot tip explants which taken from two years old V. vinifera Bozcaada Cavus Grape cv. seedlings were cultured on MS0 and Gamborg mediums for callogenesis. After healthy callus biomass have been obtained from different explant types of the grape on MS0, this callus cultures have been transferred to the MS mediums which supplemented with 1:1 mg/mL BAP:2,4-D together. Gamborg medium has been eliminated because of no callogenesis. The effects of combinations of plant growth regulators on the callus have been determined by the means of biomass levels. Subculture series was observed across four subcultures. As a result, MS medium which supplemented with BAP and 2,4-D together choosed for the callogenesis experiments and callogenesis induction has been increased %80 when we compare with MS0 medium. There are no research have been found on petiole and shoot tip explants of this cultivar which cultured on MS and Gamborg mediums for callogenesis. In our research, it has been demonstrated that different nutrient medium types and plant growth regulators have been efffective on callogenesis in different levels.

Keywords: Vitis vinifera, in vitro culture, BAP (benzylaminopurine), 2,4 D (2,4 Dichloropheno xyacetic acid)

DOI: 10.7176/JSTR/7-04-05