Morphometric Effect of Erythropoietin Used as Doping and Swimming Exercise in Female Rats in Puberty on Humerus and Femur Bones

Abstract

In this study, the effect of human erythropoietin (rhEPO) with aerobic exercise on the femur and humerus bone of female rats in the pubertal period was investigated by morphometric method. The study was performed on 40 female rats of the Spraque – Dawley genus.

The rats were divided into four groups as erythropoietin, swimming exercise with erythropoietin, swimming and sedentary. For 4 weeks, every other day all rats were injected with rhEPO (100 IU / kg, IP) 4 days a week. After the injection, the swimming group with rhEPO and the solo swimming group were swam for 30 minutes. At the end of 4 weeks, the rats were euthanized, and corpus, height and cortex and cavum medulla measurements of humerus and femur bones were done.

Statistical evaluation also indicated that there were no differences (p>0.05) between rhEPO group, swimming group and rhEPO + swimming group femur length and femur cavum medulla da sedentary group. Sedentary group was found to be thicker (p<0.05) in femoral Corpus than swimming and rhEPO group, while there was no statistical difference in femoral cortex but a numerical difference was found.

While Humerus bone length and Corpus were not significant in our study (p>0.05), although the numerical values of rhEPO + swimming group in Cortex and Cavum medullada were different compared to other groups, no statistical difference was found (p > 0.05). As a result, it is thought that long-term use of erythropoietin may cause bone development disorders.

Keywords: Doping, Erythropoietin, Exercise, Morphometry

DOI: 10.7176/RHSS/10-9-08

Publication date:May 31^st 2020   Special issue on science research