Syntheses, Characterization, and Antimicrobial Screening of N-(benzothiazol-2-yl)-2-nitrobenzenesulphonamide and its Cu(I), Ni(II), Mn(II), Co(II) and Zn(II) Complexes

Abstract

N-(benzothiazol-2-yl)-2-nitrobenzenesulphonamide (NBS2ABT) was synthesized by the condensation of 2-aminobenzothiazole and 2-nitrobenzenesulphonylchloride under reflux. Five metal complexes of Cu(I), Ni(II), Mn(II), Co(II) and Zn(II) of the ligands were synthesized. The compounds were characterized using magnetic susceptibility measurements, mass spectrometry, elemental microanalysis, UV/VIS spectrophotometry, infra red, ¹H and ¹³C nmr spectroscopies. The antimicrobial tests of the ligands and its metal complexes were carried out on both multi-resistant bacterial strains isolated under clinical conditions and cultured species using agar-well diffusion method. The multi-resistant bacterial strains used were E. coli, Proteus species, P. aeroginosa and S. aureus which were isolated from dogs. The culture species were P. aeruginosa (ATCC 27853), E. coli (ATCC 25922), S. aureus (ATCC 25923), and the fungi, C. krusei (ATCC 6258) and C. albicans (ATCC 90028). The tests were both in vitro and in vivo. The antimicrobial activities of the compounds were compared with those of Ciprofloxacin and trimethoprim-sulphamethoxazole as antibacterial agents and Fluconazole as an antifungal drug. All the compounds showed varying activities against the cultured typed bacteria and fungi used. The Lethal Concentration (LC₅₀) ranged from 5.00±0.86-618.90±30.8 ppm. These are within the permissible concentrations.

Key words: N-(benzothiazol-2-yl)-2-nitrobenzenesulphonamide, Metal complexes, Antimicrobial, Ligand