Relative Examination of Monosaccharide and Disaccharide Adopting Polarimeter and UV-Visible Spectrophotometer Instruments.

Abstract

Polarimeter and ultra violet-visible spectrophotometer instruments were used to analyze monosaccharide and disaccharide. Linear regression equation and analysis of variance (ANOVA) were applied for statistical analysis. Uv-visible spectrophotometer was more sensitive than polarimeter (R² = 0.94).  Significant difference (P<0.05) was observed between the two methods. Identification of higher percentage of specific monosaccharides was possible. This was shown by the significant difference among sugars for the methods. Uv-visible spectrophotometer and polarimetry showed least sensitivity for determination of fructose. In recent times, ethanol from bio resource is being considered as an alternative source of energy, glucose which is one of the monosaccharide of carbohydrate can be converted to ethanol, and the quantity of the ethanol produced is dependent on the initial concentration of (carbohydrate) that was fermented. Other industrial use of carbohydrates includes pharmaceutical uses, paints ink etc. It is therefore important to compare instruments which will be adopted for rapid, sensitive and accurate results on the quantity and quality of carbohydrates.

Keywords: Monosaccharide, disaccharide, Uv-spectrophotometer and Polarimeter.