Proximate Composition, Phytochemical and Elemental Analysis of Some Organic Solvent Extract of The Wild Mushroom- Ganoderma lucidum.

Abstract

Brief about the title: The crude extract powder of Ganoderma lucidum harvested from Lafia, Nassarawa State of Nigeria during the rainy season was analyzed for proximate contents, phytochemical constituents and mineral composition using various standard methods. Description of the experiment and procedures: The harvested Ganoderma lucidum was air dried at 37⁰C and grinded to powder; this was preliminary analyzed for proximate contents, phytochemical constituents. The crude powder was subjected to soxhlet extraction at 40⁰C using Methanol, Ethylacetate and N-butanol to obtain different organic solvent fractions, these were then concentrated in vacuo at 24⁰C for 48 hours to obtain different solvent extract fractions. These extracts were then analyzed for phytochemical contents using standards methods. Results: Analysis for proximate constituent showed Moisture contents was 10.54%, Total ash 5.93%, Protein 17.55%, Crude Fats 2.60%, Crude Fiber 30.25%, Carbohydrates 33.13%, and Nitrogen 23.52%. Phytochemical screening from unprocessed G.lucidum powder reveals the presence of:  Alkaloids, Flavonoids, Reducing sugars, Tannins, Cardiac glycosides, Anthraquinones, Saponins, Volatile oils and Steroids. However, variations in the presence and concentrations of these phytochemicals were observed in the partitioned portions separated by Methanol, Ethyl acetate and N-butanol. Analysis of Total ash reveals in (mg/kg) the presence of Ca (322.6), K (317.1), P (197.1), and Na (193.5) in high quantity, while C (68.2), Fe (44.6), and Zn (14.65) and Mg (8.7.0), were found to be in moderate quantity. However, Si (4.10), Mn (1.83), As (1.23), Cu (0.84), Cr (0.14), Pb (0.106), Mo (0.09), Ni (0.095), F (0.0039), Al (0.20) and Co (0.026) are very low in concentration, but Se (0.00) was completely not detected. Conclusion: The presence of these essential nutrients and minerals found in G.lucidum implies that it can be utilized for its medicinal values in healthcare delivery systems, and the medicinal importance are thus highlighted in this work.

Keywords: Ganoderma lucidum, Fruiting body, proximate analysis, Phytochemical and Mineral, Healthcare