Comparison of RP-HPLC and UV Spectrophotometric Methods for the Determination of Orphenadrine Citrate and Diclofenac Sodium in Pharmaceutical Drugs

Abstract

This paper describes the development and evaluation of a RP-HPLC and UV spectrophotometric methods to quantify Orphenadrine Citrate and Diclofenac Sodium in Tablets and Injections. RP-HPLC analysis were carried out using a C₁₈ Knauer column and a mobile phase composed of  (1% Triethylamine aqueous buffer  adjust pH=2 by H₃PO₄ 85%: Methanol: Acetonitrile) ; (35: 20: 45) v/v%, with a flow rate of 2.0 mL/min and UV detection at 195 nm. For the spectrophotometric analysis, (Ultra pure water: Methanol) ; (50: 50) v/v% was used as solvent and the wavelength of 264, 277 nm was selected for  Orphenadrine Citrate and Diclofenac Sodium, respectively the detection. Both methods were found to quantify Orphenadrine Citrate and Diclofenac Sodium in Tablets and Injection accurately. Therefore RP-HPLC and UV methods presented the most reliable results for the analyses of Tablets and Injection.

Keywords: Orphenadrine Citrate; Diclofenac Sodium; UV spectrophotometry; RP-HPLC; pharmaceutical formulations.