Curing HIV-1 Infection via in vitro Ultra-Sensitive Modification of HIV-1 Uninfected CD4+ Cells using Antiviral Agents

Abstract

Curing HIV-1 Infection via in vitro ultra-sensitive modification of Human Immunodeficiency Virus-1 (HIV-1) uninfected CD4⁺ cells is a promising hypothetical procedure that links different established clinical/laboratory tools (methods) and channeling them towards a clinical trial to achieve the ultimate goal of curing HIV-1 infection. The In vitro ultra-sensitive chemical modification of HIV-1 uninfected CD4⁺ cells using selected anti-viral agents towards curing HIV-1 infection entails a six step clinical/laboratory procedure. Isolation/purification of HIV-free CD4⁺ cells is the first step of this therapeutic procedure. In vitro CD4⁺ cell expansion/culture, ultra-sensitive chemical modification, inhibition/suppression of CD4⁺ cell proliferation, administration of chemically modulated cells to HIV-infected patient, monitoring/maintaining the physiological/biochemical processes under intensive care make up the other steps of this procedure consecutively. The milestones in this therapeutic method include increased bioavailability, minimum dosage, minimal or no side effects, shorter duration of treatment, mop-up of free plasma virions. The principal reason for this adopted method is that, the therapy overcomes the limitations posed by the current methods of managing HIV-1 infection using anti-retrovirals.

Keywords: Curing, CD4⁺ cells, HIV-1 infection, antiviral, side effect, modification