Prevalence of CTX-M Gene in Klebsiella Pneumonia Isolated from Surface Water of Tigris River within Baghdad Province

Afrah F. Abd Al-Kareem, Kadhum H. Al-Arajy, Kifah A. Jassim


The present work was conducted to study the prevalence of klebsiella pneumonia in surface water which a part of feacal coliform bacteria that consider one of  microbial pollution indicators in water, also determined their antibiotic resistance and ESBL encoding gene using PCR with specific primers for the detection of CTX-M gene. The results showed a total of 40 isolates of klebsiella pneumonia were tested for the presence of the CTX-M gene by PCR, 87.5% were carrying this gene, and also isolates were resistant to various classes of antibiotics. Water and sewage are the ones, which have been identified as reservoirs of enteric bacteria for spread of resistance factors, wastewater treatment plant did not show a satisfactory efficacy in removing pathogenic microorganisms, allowing for the dissemination of multiresistant bacteria into the environment and this can result in routes of dissemination of multiresistant bacteria and their genes of resistance into the environment, thus contaminating water resources, and having serious negative impact on public health.

Keywords: CTX-M, k. pneumonia

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ISSN (Paper)2224-7181 ISSN (Online)2225-062X

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