Molecular Characterization of Restriction Endonuclease Extracted from Local E.coli Isolate

Abstract

An endonucleases enzyme named EcoRHI has been purified partially from one of pathogenic E.coli isolate about 87-fold with endonuclease recovery about 12.6% . The purification steps included ammonium sulfate precipitation with 50% saturation ratio, and the enzymatic solution reclaimed it through Sephadex G-100 column .The SDS-PAGE analysis that showed molecular weight of EcoRHI enzyme is 45 KDa. The purified endonuclease was able to digest λ-DNA into two fragments.