Evaluation of Anti Oxidant and Anti inflammatory Activity of Banana Peels

Abstract

In the preset study, synthesis iron oxide nanoparticles (Fe3O4-NPs) were synthesized using a rapid, single step and completely green biosynthetic method by reduction of ferric chloride solution with banan peel water extract containing sulphated polysaccharides as amain factor which acts as reducing agent and efficient stabilizer. The structural and properties of the Fe3O4-NPs were investigated by X-ray diffraction, FTIR Fourier transforminfrared spectroscopy and  Ultra violate UV. The diameter of iron nanoparticles was predominantly found within the range 15-35 nm. we evaluated the anti-inflammatory effect of Fe3O4-NPs by in vitro method by using membrane stabilization test and protein denaturation test. Membrane stabilization test was done by using human red blood cells (HRBCs). Protein denaturation test was done by using bovine serum albumin (BSA). The results revealed that (Fe3O4-NPs) was capable of rendering membrane stabilization by inhibiting the hypotonically-induced hemolysis of HRBCs in dose-dependentmanner).

Keywords: Nanoparticles, Fe3O4-NPs, X-ray, HRBCs, BSA.