Liposome Mediated Transfection in Eukaryoic Cell: An Overview

Abstract

In Gene therapy Nucleic acids can be inserted into human cells to provide enhancement or reduction of protein expression for the prevention, treatment or elimination of the problem. For this to happen foreign gene should be inserted in to the cells using different mechanisms. The commonly used ways of gene delivery in to eukaryotic cells are of viral and non viral delivery systems. The non viral delivery are of different types of which the liposome mediated tranfection is the most widely used. Liposome mediated transfect ion represent approved non-toxic biocompatible nanoparticles for the application of medicine. Excellent biocompatibility, low immunogenicity, delivery of large piece of nucleic acid and simplicity of handling are the major advantages one has to see in liposome transfection. But the presence of positive charge on liposomes vectors which may favour nonspecific interaction with negatively charged serum protein, enzymes and causing in to decreased cell adhesion, hemolysis and low transfection are among the disadvantages. In this short review different types of liposomes and their reagents are highlighted. Generally the use liposome in the area of biomedicine (not only for gene delivery) gives a paramount relief specially in this era of drug resistance, vaccine failure, threatening emerging and re-emerging pandemics in the globe.
Keywords:-Liposome, Gene therapy, Transfection, Eukaryotic cell